Supplementary MaterialsS1 Fig: Multiphoton microscopy of day 8 X31-OVA contaminated trachea with OT-I Compact disc8+ T cells

Supplementary MaterialsS1 Fig: Multiphoton microscopy of day 8 X31-OVA contaminated trachea with OT-I Compact disc8+ T cells. (reddish colored). Outer collagen sheath has an optical sign through second harmonic era (white).(MP4) ppat.1005881.s002.mp4 (12M) GUID:?829D2D9B-27E0-4A75-91CE-887AA161FEE2 S2 Film: Prolonged focus videos of day time 6 CD8 T cell motility in the flu contaminated trachea. Adoptively moved OT-I-GFP Compact disc8+ T cells (green) are imaged by multiphoton intravital microscopy after disease with influenza X31-OVA-I as referred to in components and methods. The films show types of the prolonged focus videos, used from a genuine perspective in the trachea peering through, utilized to create the motility guidelines for times 6C10 after disease.(MP4) ppat.1005881.s003.mp4 (4.2M) GUID:?42A7DCD9-1CF7-49BD-A2BD-A60B19338BF6 S3 Film: Extended focus video clips of day 7 CD8 T cell motility in the flu infected trachea. Adoptively moved OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples of the extended focus Eletriptan hydrobromide videos, taken from a point of view inside the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s004.mp4 (2.7M) GUID:?DB71812D-3198-43CF-A908-DC57F381DBCD S4 Movie: Extended focus videos of day 8 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as Eletriptan hydrobromide described in materials and methods. The movies show examples of the extended focus videos, taken Eletriptan hydrobromide from a point of view inside the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s005.mp4 (2.9M) GUID:?08993F05-2F3B-49B0-8B6B-BBBB54EAD012 S5 Movie: Extended focus videos of day 9 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples of the extended focus videos, taken from Eletriptan hydrobromide a point of view the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s006.mp4 (4.7M) GUID:?B4A2EF23-BC2D-46B6-8799-D182868D3C83 S6 Movie: Extended focus videos of day 10 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples of the extended Bmp1 focus videos, taken from a point of view inside the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s007.mp4 (2.0M) GUID:?37819C40-0CBA-4F49-94B1-FCE830A3DD3D S7 Movie: Extended focus videos of day 14 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The films show types of the prolonged focus videos, used from a genuine perspective beyond your trachea peering through, utilized to create the motility guidelines for times 6C10 after disease.(MP4) ppat.1005881.s008.mp4 (417K) GUID:?CDBB5ADF-DF68-4779-8A8A-958C2D1F7B94 S8 Film: 3D animation of day time 6 Compact disc8 T cell motility in the flu Eletriptan hydrobromide contaminated trachea. Adoptively moved OT-I-GFP Compact disc8+ T cells (green) are imaged by multiphoton intravital microscopy after disease with influenza X31-OVA-I as referred to in components and methods. Revolving 3D images had been reconstructed through the prolonged focus videos showing the lateral perspective from the cells to illustrate depth in the localization of T cells (green) in accordance with the external collagen sheath (white).(MP4) ppat.1005881.s009.mp4 (12M) GUID:?95653F53-F469-43CD-8332-C49387084924 S9 Film: 3D animation of day 7 CD8 T cell motility in the flu infected trachea. Adoptively moved OT-I-GFP Compact disc8+ T cells (green) are imaged by multiphoton intravital microscopy after disease with influenza X31-OVA-I as referred to in components and methods. Revolving 3D images had been reconstructed through the prolonged focus videos showing the lateral perspective from the cells to illustrate depth in the localization of T cells (green) in accordance with the external collagen sheath (white).(MP4) ppat.1005881.s010.mp4 (6.4M) GUID:?E43E9BC6-1EF1-46C6-90F8-F9EE62AD124A S10 Film: 3D animation of day 8 CD8 T cell motility in the flu contaminated trachea. Adoptively moved OT-I-GFP Compact disc8+ T cells (green) are imaged by multiphoton intravital microscopy after disease with influenza X31-OVA-I as referred to in components and methods. Revolving 3D images had been reconstructed through the prolonged focus videos showing the lateral stage.